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gapA [2017-08-02 08:59:02]
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gapA [2017-08-02 08:59:02]

glyceraldehyde 3-phosphate dehydrogenase, NAD-dependent, glycolytic enzyme, forms a transhydrogenation cycle with GapB for balancing of NADPH
locus
BSU33940
pI
5.03
mw
35.68 kDa
protein length
335 aa Sequence Blast
gene length
1005 bp Sequence Blast
function
catabolic enzyme in glycolysis
product
glyceraldehyde 3-phosphate dehydrogenase
essential
Yes
ec
1.2.1.12
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • Gene

    Coordinates
    3,481,698 → 3,482,705

    Phenotypes of a mutant

  • Essential [Pubmed|17114254]

    • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate phosphate NAD = 3-phospho-D-glyceroyl phosphate NADH (according to Swiss-Prot)
  • This reaction is part of the glycolysis.

    • Protein family

  • glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|C1B89BD3DD27CD566018558D3A7845FB451056F0|GapB]

    • Kinetic information

  • Michaelis-Menten [Pubmed|10799476]

    • Modification

  • phosphorylated on Arg-199 [Pubmed|22517742]
  • Phosphorylation on (Ser-148 OR Ser-151 OR Thr-153 OR Thr-154) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed1], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed2]
  • Reversible thiol modifications after exposure to toxic quinones [Pubmed|18673455]
  • Cys152-Cys156 form intramolecular disulfide in response to disulfide stress (diamide, NaOCl-stress) [Pubmed|21749987]

    • [SW|Cofactors]

  • NAD (does not accept NADP ) [Pubmed|10799476]

    • Structure

  • [PDB|1GD1] (from ''Geobacillus stearothermophilus'')
  • [PDB|1NQO] (from ''Geobacillus stearothermophilus'', mutant with cys 149 replaced by ser, complex with NAD und D-Glyceraldehyde-3-Phosphate)

    • [SW|Localization]

  • cytoplasm (Homogeneous) [Pubmed|16479537] [Pubmed|14600241]
  • loosely membrane associated [Pubmed|18763711]

    • Additional information

  • GAP dehydrogenases from different sources (incl. ''Geobacillus stearothermophilus'') were shown to cleave RNA ([Pubmed|12359717])
  • Moreover, mutations in ''gapA'' from ''B. subtilis'' can suppress mutations in genes involved in DNA replication ([Pubmed|17505547]).
  • extensive information on the structure and enzymatic properties of GapA can be found at [http://www.proteopedia.org/wiki/index.php/Glyceraldehyde-3-Phosphate_Dehydrogenase Proteopedia]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
    description
    [Pubmed|11489127]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489127], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]: repression, [Pubmed|11489127], in [regulon|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR regulon]

    • regulation

  • expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR] [Pubmed|11489127]
  • the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
    • view in new tab

    genes
    [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]
    description
    [Pubmed|11489127]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|11489127], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]: repression, [pubmed|11489127], in [regulon|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR regulon]

    • regulation

  • expression induced by glucose (10 fold) ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]) [Pubmed|12850135,12622823]
  • the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
    • view in new tab

    Biological materials

    Mutant

  • GP592 (''gapA''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP597 (''gapA''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP703 (''gapA''::''cat'' ''[gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB]''::''spec''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GM1501 (under p(spac) control), available in [SW|Stephane Aymerich]'s lab
  • 1A1003 ( ''gapA''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1003&Search=1A1003 BGSC]

    • Expression vector

  • pGP1424 (expression in ''B. subtilis'', in [SW|pBQ200]) (available in [SW|Jörg Stülke]'s lab)
  • pGP90 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab), [pubmed|19193632]
  • pGP704 (N-terminal His-tag, in [SW|pWH844]) (available in [SW|Jörg Stülke]'s lab)

    • lacZ fusion

  • pGP506 (in [protein|search|pAC7]), pGP512 (in [protein|search|pAC6]) (available in [SW|Jörg Stülke]'s lab)

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html homepage]

    • References

    Reviews

  • 11163353,27750368,28271485

    • Original publications

  • 22517742,23420519,22740702,23034808,21815947,21749987,12850135,19193632,18673455,20444087,17726680,16479537,12622823,12359717,10799476,17505547,11489127,12123463,17218307,12634343,17142398,17114254,10559165,15378759,27449348